過氧化物酶 來源于辣根, RZ 1.0,≥85 units/mg dry weight
Product Name： Peroxidase from horseradish
別名:HRP
CAS號:9003-99-0
介紹： Peroxidase (HRP) is a hemoprotein catalyzing the oxidation by hydrogen peroxide of a number of substrates such as ascorbate, ferrocyanide, cytochrome c and the leuco form of many dyes. HRP has a molecular weight of 40 kDa and an optimum pH of 7.0.
用途： Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana). It is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. Horseradish peroxidase is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. Product P8125 has been used to study mitochondrial H2O2 release.
貯存： 儲存溫度-20°C
生化和生理學機理： When incubated with a substrate, horseradish peroxidase produces a coloured, fluorimetric, or luminescent derivative of the labeled molecule, allowing quantification. Horseradish peroxidase has been shown to slightly reduce the level of inhibition in a cydAB mutant. HRP reacts with hydrogen peroxide (H2O2) to form a [HRP-H2O2] complex which can oxidize a wide variety of hydrogen donors.
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