胃蛋白酶 來源于豬胃, ≥2,500 units/mg dry weight
Product Name： Pepsin from Porcine Stomach
別名:胃液素 來源于豬胃;胃蛋白酶 A
CAS號(hào):9001-75-6
介紹： Pepsin is an acidic protease. Its inactive zymogen precursor, pepsinogen, is produced in the stomach mucosa. There are several pepsins designated A, B, C, and D. Pepsin A, the major component, has a molecular weight of 35,000 daltons and an optimum pH of approximately 1.0 for substrates such as casein or hemoglobin if the substrate is native protein. Pepsin cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine and tyrosine. It will not cleave at bonds containing valine, alanine or glycine. Pepsin is assayed based on the method of Anson J. Gen. Physiol., 22, 79 (1938) using hemoglobin as the substrate. Pepsin is unstable above pH 6.
用途： 用于產(chǎn)生抗體的 F(ab′)2 片段。
貯存： 儲(chǔ)存溫度2-8℃
生化和生理學(xué)機(jī)理： 優(yōu)先裂解：P1 和 P1′ 位的疏水性和芳族殘基。降解胰島素 β 鏈中的 Phe-Val、Gln-His、Glu-Ala、Ala-Leu、Leu-Tyr、Tyr-Leu、Gly-Phe、Phe-Phe 和 Phe-Tyr 鍵
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