5,5＇二硫代雙(2-硝基苯甲酸), 98%
Product Name： 5,5＇-Dithio bis-(2-nitrobenzoic acid)
別名:雙(3-羧基-4-硝基苯基)二硫，6,6ˊ-二硝基-3,3ˊ-二硫代苯甲酸 ;DTNB,Ellman’s Reagent;二硫代硝基苯甲酸
CAS號(hào):69-78-3
分子式:C14H8N2O8S2
分子量:396.35
介紹： Ellman's Reagent (DTNB) reacts with sulfhydryl groups to yield a colored product, providing a reliable method to measure reduced cysteines and other free sulfhydryls in solution. Ellman's Reagent (5,5'-dithio-bis-[2-nitrobenzoic acid]) is used to estimate sulfhydryl groups in a sample by comparing to a standard curve of a sulfhydryl-containing compound such as cysteine. Alternatively, sulfhydryl groups can be assayed using the extinction coefficient of TNB (14,150M-1cm-1 at 412nm). Ellman's Reagent has been used for applications other than sulfhydryl estimation, such as for determining alkylthiols by HPLC using a pre-column derivatization procedure and to study thiols in the active site of several enzymes. Highlights： ?Sulfhydryl assay – reacts quantitatively with free (reduced) sulfhydryl groups (–SH) to yield detectable TNB product ?Colorimetric detection – colored product enables spectrophotometric measurement in cuvettes or microplates (λmax = 412nm; ε = 14,150/M?cm) ?Proven method – well-characterized chemistry provides quantitation of peptide or protein sulfhydryl groups using extinction coefficient calculation or by comparison to a cysteine standard
用途： 巰基測(cè)定，是測(cè)定蛋白質(zhì)、多肽和組織中巰基的靈敏試劑。也用于有機(jī)磷農(nóng)藥中毒的監(jiān)測(cè)（膽堿酯酶測(cè)定）。
熔點(diǎn)： 240～245℃
貯存： 陰涼干燥處保存;儲(chǔ)存溫度2-8°C
敏感性： 熱敏感
|
?您的位置：
