焦碳酸二乙脂(DEPC), 98%
Product Name: Diethyl pyrocarbonate
別名:焦碳酸二乙酯 ;氧二甲酸二乙酯
CAS號(hào):1609-47-8
分子式:C6H10O5
分子量:162.14
介紹: Diethyl Pyrocarbonate has been shown to inhibit ryanodine binding to ryanodine/Ca2+ receptor channels in skeletal muscle in a dose- and time-dependent manner and increases the Ca2+ permeability of SR vesicles. This compound could be useful for specific inactivation of nucleases during isolation of undegraded polynucleotides. Diethyl Pyrocarbonate also inhibits PAF Acetylhydrolase (platelet-activating factor acetyl hydrolase), and has been used as a probe for the topography of 5.8S rRNA in yeast ribosomes.
用途: 蛋白質(zhì)組氨酸和酪氨酸的修飾試劑。也是RNA酶的化學(xué)修飾劑,它和RNA酶的活性基團(tuán)組氨酸的咪唑環(huán)反應(yīng)而抑制RNA酶活性。
沸點(diǎn): 93-94°C
特定比重: 1.121
折光率: 1.396-1.4
貯存: 儲(chǔ)存溫度 2-8°C
敏感性: 對(duì)濕度敏感
可溶性: Soluble in chloroform, ethanol (50% w/w), esters, ketones, and hydrocarbons.Slowly soluble in water with hydrolytic decomposition yielding ethanol and CO2.
生化和生理學(xué)機(jī)理: 在溶液中濃度約為 0.1% (v/v) 時(shí)可使 RNase 失活，從而防止 RNA 被降解。
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