SJB2-043
Product Name： SJB2-043
CAS號:63388-44-3
分子式:C17H9NO3
分子量:275.26
貯存： 儲存溫度-20°C
可溶性： 25°C： DMSO
生化和生理學(xué)機(jī)理：
Description：
IC50 Value： 0.544 uM (USP1) [1]
SJB2-043 is one of analogs/derivatives of C527, which is a novel and potent USP1 (ubiquitin-specific protease 1) inhibitor.
Cells were treated with SJB2-043 for 24 hrs and cell extracts were incubated with HVIP(LA)Ub-VS, followed by immunoblotting with anti-USP1 or anti-HA antibodies. Consistent with our previous studies and the most recent studies by Piatkov et al (24, 31, 33), USP1 in the untreated cells was detected on western blot as a doublet; full length USP1 and an auto-cleaved USP1 with only N-terminus. The Ub-USP1 conjugates were generated in the untreated cell lysates and were detected by an increase in molecular weight of USP1. However, the formation of the Ub-USP1 conjugate was inhibited by the addition of SJB2-043. Ub-aldehyde, a potent known nonspecific DUB inhibitor, caused a complete loss of USP1-Ub-VS conjugates in this competition assay. SJB2-043 inhibited the Ub-VS labeling of a limited number of
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